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. 2012 Sep 3;198(5):815–832. doi: 10.1083/jcb.201201050

Figure 7.

Figure 7.

Six1 gene disruption increases SCs self-renewal, but does not perturb the orientation of SC divisions. (A) FACS-sorted SCs were plated ex vivo and fixed after the first division. Typical doublets of sister SCs with Pax7+/+ or Pax7+/− gene signature are shown. (B) Six1KO SCs have higher self-renewal potential. (C) Immunolocalization of Pax7 and Ki67 proteins on myofibers separated from 4-d regenerating EDL muscles. (D) Quantification of SC division orientation. Six1 gene disruption does not have an impact on the rate of planar-to-perpendicular divisions. (E) EDL myofibers were separated from 4-d regenerating EDL muscles, and immunolocalized for Pax7 and Myf5 protein expression. Six1 gene disruption does not have an impact on the Myf5-negative satellite stem cell population. (F) FACS-sorted SCs separated on the basis of Myf5-Cre–driven reporter fluorescence and plated ex vivo. Immunolocalization of Pax7 and YFP proteins on YFP− (stem) and YFP+ (committed) myoblasts. (G) qRT-PCR analysis indicated expression of Six1 transcripts by YFP+ and YFP− SCs and myoblasts. Error bars indicate standard deviations. *, P < 0.01. Bars: (A) 10 µm; (C and F) 50 µm.