Fig. 2.

Characterization of Rtel1-V5 protein expression in pCX-Rtel1-V5 mice. a–f IHC with anti-V5 antibody on mouse tissues collected from 2 months old pCX-Rtel1-V5 mice. a liver; b lung; c heart; d kidney; e intestine; f pancreas. g western blot with anti-V5 antibody on mouse tissue lysates harvested from pCX-Rtel1-V5 mice. 1 liver; 2 intestine; 3 liver from wild-type control. Arrow indicates a major detected protein band with 150 Kd that matches with the predicted molecular weight of full length Rtel1-V5 protein