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DA and DOPA mediate formation of α-syn soluble oligomers. α-syn was incubated alone or in the presence of DA (100 µM) or DOPA (100 µM) in Tris-HCl, pH 7.8 at 37°C for 24 h. Co-incubation of α-syn with DA or DOPA enhances α-syn oligomerization in vitro. The reaction mixture was separated by SDS-PAGE, and α-syn monomer and oligomer are detected by silver staining.
