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. 2012 Aug 22;8(7):1062–1074. doi: 10.7150/ijbs.4488

Figure 2.

Figure 2

Figure 2

Figure 2

TGF-β1 induced expression of SMC markers (α-SMA, h1-calponin and SM22α) but suppresses expression of EC marker (Tie-2) in PDL-derived EPC-like fibroblastic cells. In A and B, SCDC2 cells were cultured for 3 days in growth medium supplemented with 5% FBS in the presence of the indicated concentrations of TGF-β1. Some of the cells were treated with the TGF-β receptor inhibitor SB-431542 (10 μM), which was added to the culture 30 min before the TGF-β administration. The relative mRNA expression levels of SMC markers (α-SMA, h1-calponin and SM22α) (A) and EC markers (Tie-2 and VE-cadherin) (B) in the cells were analyzed by qRT-PCR as described in Materials and Methods. Data represent the mean ± SD (n = 3). *P <0.05, **P <0.02, and ***P <0.01 were considered significant. In C, cells were cultured for 3 days in growth medium supplemented with 5% FBS as a control (a, e, and i) or in medium with 0.3 or 3 ng/mL TGF-β1 (b, c, f, g, j, and k). Some of the cells were treated with the TGF-β receptor inhibitor SB-431542 (10 μM), which was added to the culture 30 min before the TGF-β (3 ng/mL) administration (d, h, and l). The cells were immunostained with anti-α-SMA (a-d) (red), anti-h1-calponin (e-h) (red), and anti-Tie-2 antibodies (i-l) (red), and then labeled with Alexa Fluor® 488 phalloidin (green) as described in Materials and Methods. Scale bar, 50 μm.