Fig. 2.
Western-blot analysis of cytosolic (a, b) and membrane (c, d) fractions from proliferating and differentiated (6-day RA-treatment) SHSY-5Y cells, respectively. Naïve (marked as ‘n’) and polyclonal cells (marked as ‘p’) were included in the analysis. Each lane contains 14 μg of soluble protein. Loading controls for the cytosolic and membrane fractions were β-actin and lamp2 respectively. All four blots were processed in parallel, using identical conditions and exposure times.