Fig. 2.

IL-1β-dependent EVT motility. (A) Migration of first trimester primary EVT through fibronectin-coated transwells in the absence or presence of human recombinant IL-1β and/or IL-1Ra. Invasion of CTK-7-positive trophoblasts was evaluated as described in Materials and methods. Bar graphs represent mean values ± S.D. of three experiments performed in duplicates. * indicates p < 0.05; ns, not significant; (B) IL-1β induced migration of primary EVT in first trimester villous explant cultures cultivated on collagen I. Representative pictures are shown (200 fold magnification). Migration of trophoblasts was analysed as mentioned in Materials and methods. Bar graphs represent mean values ± S.D. of untreated (n = 60) and IL-1β-stimulated (n = 60) villous explants isolated from five different placentae. * indicates p < 0.05.