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. 2012 Sep;33(9):696–703. doi: 10.1016/j.placenta.2012.05.008

Fig. 4.

Fig. 4

IL-1β-stimulated secretion of uPA and PAI proteins in first trimester villous explant cultures and primary EVT. Western blot analyses of supernatants and quantification were done as described in Materials and methods. (A) Western blots showing IL-1β-dependent expression of uPA, PAI-1 and PAI-2 in first trimester villous explant cultures in the absence or presence of IL-1Ra. Bar graphs show mean values ± S.D. of untreated and IL-1β- and/or IL-1Ra-stimulated explant cultures isolated from three different placentae, n = 36 for each condition; ns, not significant, * indicates p < 0.05. (B) Western blot analyses of uPA, PAI-1 and PAI-2 in primary EVT after 48 h of IL-1β treatment. Two different EVT cell pools are shown. Bar graphs represent mean values ± S.D. of three different primary EVT cell isolations, * indicates p < 0.05.