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Diagrammatic view of the 3D culture system. rASCs were seeded on the upper side of the membrane of a Millicell insert coated with 0.10% collagen type IV. To create an ALI culture system, the inducing medium in basolateral compartment was raised to reach the level of the membrane, then the cells were exposed to the air with 5% CO2 with 95% relative humidity while fed from the medium underneath. rASCs, rabbit adipose-derived stem cells; ALI, air–liquid interface.
