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. 2012 Jun 11;18(17-18):1760–1770. doi: 10.1089/ten.tea.2011.0424

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Copyright 2012, Mary Ann Liebert, Inc.

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FIG. 5. — (a) Expression of epithelial-specific genes (cytokeratin 19, cytokeratin 13, and involucrin) and α-SMA in rASCs cultured under different conditions determined by western blot analysis. (b) Histograms show the relative intensity of cytokeratin 19, cytokeratin 13, involucrin, and α-SMA normalized to GAPDH (expressed as the ratio of cytokeratin 19, cytokeratin 13, involucrin, or α-SMA to GAPDH). It was revealed that the expression of epithelial-specific markers were enhanced in rASCs treated with RHE medium (cytokeratin 19 and cytokeratin 13) and RHEHK medium (cytokeratin 19, cytokeratin 13, and involucrin) compared with the undifferentiated cells in rASCs group, while the expression of α-SMA was decreased in both RHE-treated and RHEHK-treated group compared with that in the rASCs group. Further, more significant enhancement in cytokeratin 19 and cytokeratin 13 expression in the RHEHK-treated group was observed compared with that in the RHE-treated group. *p<0.01 compared with rASCs group; ^§p<0.05 compared with rASCs group; ^#p<0.05 compared with RHE-treated group. n=3. α-SMA, alpha-smooth muscle actin.