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. 2012 Jul 25;32(30):10226–10237. doi: 10.1523/JNEUROSCI.0007-12.2012

Figure 1.

Figure 1.

Production of α6-GFP BAC transgenic mice and α6* nAChR expression in visual system. A, Construction of α6-GFP BAC transgene. A BAC containing the full Chrna6 gene was modified by recombineering to allow expression of a variant α6 nAChR subunit with GFP fused in-frame within the M3–M4 intracellular loop. B, C, α6* nAChR expression in retina. Whole-mount retinal sections were prepared from α6-GFP transgenic mice and stained with anti-GFP antibodies. GFP(+) cells in the GCL were imaged with confocal microscopy at 10× (B1), 60× (B2), and 480× (B3). Retinal cross sections were prepared from α6-GFP transgenic mice (C), followed by anti-GFP staining (brown color) and hematoxylin counterstaining to label nuclei. α6 immunoreactivity was confined to the GCL. D, E, α6* nAChR expression in visual thalamus and SC. Brains from α6-GFP transgenic mice were fixed, sectioned at 50 μm (coronal), and stained with anti-GFP antibodies. dLGN (D) and SC (E) stained positive for α6* nAChR expression. PL, photoreceptor layer; ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer.