Figure 3.

Normal α6* nAChR function in α6-GFP transgenic mice. A, Normal Rb⁺ efflux in α6-GFP transgenic mice. A crude synaptosomal pellet was prepared from α6-GFP and their nontransgenic littermates, followed by Rb⁺ loading and stimulation of Rb⁺ efflux by ACh application. Rb⁺ efflux sensitive to αCtxMII (which includes α6-dependent efflux) is shown for 0.3, 3, and 30 μm ACh. B, C, Normal DA release in α6-GFP transgenic mice. ST (B) and OT (C) were dissected from α6-GFP and their nontransgenic littermates, followed by preparation of synaptosomes. Synaptosomes were loaded with [³H]-DA and stimulated with a range of ACh concentrations (10, 30, 100, and 300 nm and 1 and 3 μm) in the presence and absence of αCtxMII. Data were fitted to the Hill equation, and αCtxMII-sensitive concentration response curves are shown for ST (B) and OT (C).