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. 2012 Jun 29;2(4):455–467. doi: 10.1002/brb3.69

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© 2012 The Authors. Published by Wiley Periodicals, Inc.

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Nr4a1. (a) schematic diagram showing the Nr4a1 gene, orientated reading forwards. Exons are indicated by the numbered boxes. The red box highlights the region assessed for DNA methylation in this study. The green boxes indicate CpG islands in this region. Individual CpG sites are represented by black lines: gray lines show CpG sites unable to be assessed by our method. CpG units (individual sites or groups of sites) are indicated underneath the CpG sites. These unit numbers correspond to the graph showing mean (±SEM) methylation percentages. (b–c) Percentage methylation of a CpG region upstream of the Nr4a1 gene. (d) Maternally separated C57BL/6J males have decreased methylation at CpG Unit 2 (P < 0.01).