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SK-Hep1 cells produced vimentin in rt-PCR analysis of mRNA extracted after 12 hours of culture in admixture with hMSCs. This EMT marker expression was significantly inhibited by both OPN-R3-APT (p<0.0001) and RGD blockade (p<0.0001). Vimentin expression was not inhibited in negative control assays with media additives of mutant OPN-R3-APT (p=NS) or RGD blockade (p=NS).
