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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Mol Cancer Res. 2011 Dec 15;10(2):230–238. doi: 10.1158/1541-7786.MCR-11-0296

Figure 1.

Figure 1

A. Western blotting for the detection of HIF-1α in LNCaP cells exposed to normoxia (N) or hypoxia (H) for 24 hours. Detection of GAPDH was used for normalization of protein loading; B–C. LNCaP cells exposed to hypoxia (H) for 7 days, compared to cells exposed to normoxia (N), assessed by phase contrast microscopy (B) and by immunoperoxidase staining (brown) for the detection of NSE (C); D–E. Representative immunoblot for the expression of NSE (D) and β3-tubulin (E) in LNCaP cells exposed to normoxia (N) and hypoxia (H) for 7 days. Detection of GAPDH was used for normalization of protein loading. F–G. Densitometric analysis of three independent experiments for the detection of NSE (F) and β3-tubulin (G); values are expressed as mean ± SE. H. Neurogenin (Ngn)3 mRNA expression in LNCaP cells exposed to normoxia (N) and hypoxia (H), at different time points, assessed by quantitative real time PCR.

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