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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Mol Cancer Res. 2011 Dec 15;10(2):230–238. doi: 10.1158/1541-7786.MCR-11-0296

Figure 2.

Figure 2

A–B. Notch 1 (N1), N2, Jagged (J)1, J2, Delta-like (Dll)-1, Dll-4 mRNA expression in LNCaP cells exposed to normoxia and hypoxia for 7 days, assessed by quantitative real time PCR. Data are expressed as fold expression of mRNA levels under hypoxia, normalized to normoxia level (equal 1). C. Western blotting for the detection of transmembrane N1 and N2 proteins in LNCaP cells exposed to normoxia (1), hypoxia (2) and hypoxia + DAPT [20 μM] (3), for 7 days. D. Expression of Notch target genes, Hes1 and Hey1, mRNA in LNCaP cells exposed to normoxia and hypoxia for 7 days, assessed by quantitative real time PCR. Cells treated with the γ-secretase inhibitor DAPT [20 μM] were used as control for inhibition of Notch signal.

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