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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Mol Cancer Res. 2011 Dec 15;10(2):230–238. doi: 10.1158/1541-7786.MCR-11-0296

Figure 3.

Figure 3

A–C. LNCaP cells virally transduced with LacZ or with a dominant negative form of Hes1 (dnHes1), in frame with a HA-tag, were analyzed for Ngn3 and CGA mRNA expression 4 days after transduction, by quantitative real time PCR (A–B) and for NSE and β3-tubulin protein expression, by Western blotting (C). Detection of HA-tag was used to determine the expression of the transduced dnHes1 construct. Detection of GAPDH was used for normalization of protein loading. D–E. LNCaP cells virally transduced with LacZ or with a constitutively active form of N1 (caN1), in frame with a V5-tag, were analyzed for CGA mRNA expression 3 days after transduction, by quantitative real time PCR (D), and for NSE and β3-tubulin protein expression, by Western blotting (E). Detection of V5-tag was used to determine the expression of the transduced caN1 construct. Detection of GAPDH was used for normalization of protein loading.

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