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. Author manuscript; available in PMC: 2012 Sep 4.
Published in final edited form as: Immunity. 2010 Aug 5;33(2):241–253. doi: 10.1016/j.immuni.2010.07.015

Figure 1. SAP deficiency severely compromises the development of Tfh cells.

Figure 1

WT or Sh2d1a−/− (SAP KO) OT-II cells were transferred to congenic recipients that were then immunized with OVA plus Alum i.p. (A) the proportion of OT-II cells in the spleen was determined at various times (Mean ± SEM, n=5–14). (B–C) Cells were stained for CXCR5 and PD1 and the number of CXCR5hiPD1hi cells was determined (Mean ± SEM, n=5–14). (C) Contour plots show CXCR5 and PD1 expression on OT-II cells on days 3 (left panel) and 7 (right panel) post-immunization. Cells were stained for CCR7 (D) or ICOS (E). Histograms show CCR7 or ICOS expression on endogenous CD4+ T cells (grey) and OT-II cells (red) after 3 (upper panel) or 7 (lower panel) days. Graphs show relative change in expression of CCR7 and ICOS on OT-II cells compared to endogenous CD4+T cells (Mean ± SEM, n=7–8). (F) Immunofluorescence staining of spleen sections was carried out to determine the positioning of OT-II T cells (CD45.2+) within the follicle (IgD) and T cells zone (CD4) after 3 (left panel) or 7 days (right panel). (G) Further staining confirmed positioning within the GC (PNA) at day 7.