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. Author manuscript; available in PMC: 2012 Sep 4.
Published in final edited form as: Immunity. 2010 Aug 5;33(2):241–253. doi: 10.1016/j.immuni.2010.07.015

Figure 2. Rescuing germinal centre formation does not facilitate Tfh cell development from SAP-deficient CD4+ T cells.

Figure 2

CD45.1-congenic mice were transferred with CD45.2+ OT-II cells. Mice received either Thy1.1− WT OT-II or Thy1.1+ SAP KO OT-II alone or a combination of equal numbers of Thy1.1− WT OT-II or Thy1.1+ SAP KO OT-II and were then immunized i.p with OVA plus Alum. The responses were examined 7 days later. (A) The proportion of IgG1+GL7+ (GC) B cells in recipient spleens. (B) The proportion of OT-II cells from each donor was determined – WT OT-II: filled bars, SAP KO OT-II: open bars. (C) The proportion of CXCR5hiPD1hi Tfh cells from each donor was determined. All graphs show Mean ± SEM, n=4 (D) Contour plots show expression of CXCR5 and PD1 on OT-II cells of each genotype.