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. Author manuscript; available in PMC: 2012 Sep 4.
Published in final edited form as: Immunity. 2010 Aug 5;33(2):241–253. doi: 10.1016/j.immuni.2010.07.015

Figure 4. B cell activation via CD40 is not required for Tfh development.

Figure 4

Chimeras were generated as described in Experimental Procedures (see also Figure S4A) using 80:20 mix of µMT:Cd40−/− BM (to generate mice with B cells lacking CD40) or B6: Cd40−/− BM (controls). Thy1.1+ WT OT-II cells were transferred into the chimeras, which were then given OVA plus Alum i.p. at day 0. On day 3 some mice received additional OVA peptide i.v. and the mice were sacrificed on day 7 for analysis. (A) Reconstitution was determined by staining B cells (B220+) and DC (CD11c+) for CD40 expression. (B) The proportion of total OT-II cells in the spleen. (C) The proportion of CXCR5hiPD1hi Tfh OT-II cells was determined by (D) staining for CXCR5 and PD1 on OT-II cells. (E) Proportion of GC B cells as assessed by staining for expression of GL7 and Fas. (F) Percentage of cells OT-II cells that are CD127lo. All plots show Mean ± SEM, n=7–14.