Table 1.

Calibration parameters and settings for the evaluated IMS system.

Calibration parameter	Value
Microscope objective, IMS relay objective	5×, 2.5×
Image mapper dimensions	~23.4 ×26 mm2, 65 μm facets
Slit size, slit size after magnification	5 μm × 5 mm, 62.5 μm × 62.5 mm
Spatial samples perpendicular, parallel to facets (x, y)	667, 120
Motorized stage step size (x, y)	3 μm, 16 μm
Spectral samples (x, y)	1 (determines facet order), 4 (determines dispersion)
Total images recorded, total disk space compressed	1147 images, ~100 MB