Table 1.

FVIII and FV mutants

Residues	FVIII mutants			FV mutants
	Spike 1	Spike 3		Spike 1	Spike 3	Spike 4
	2199/2200	2251/2252		2063/2064	2116/2117	2182/2183
Name	Amino acids		Name	Amino acids
FVIIIWT	Met Phe	Leu Leu	FVWT	Trp Trp	Leu Ser	Gln Ser
FVIIIWW	Trp Trp*	Leu Leu	FVMF	Met* Phe*	Leu Ser	Gln Ser
FVIIILS	Met Phe	Leu Ser*	FVLL	Trp Trp	Leu Leu*	Gln Ser
FVIIIWW/LS	Trp Trp*	Leu Ser*	FVMF/LL	Met* Phe*	Leu Leu*	Gln Ser
			FVMTTS/Y	Trp Trp	Met Thr (Thr Ser)*†	Gln Tyr

WT denotes wild-type FVIII with the native sequence.

^*Amino acids that have been changed from the wild-type sequence.

^†Substitutions were also made for native residues Ser2118/Glu2119 (see Figure 5) for mutant FV_MTTS/Y.