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. Author manuscript; available in PMC: 2013 Jul 17.
Published in final edited form as: Anal Chem. 2012 Jun 28;84(14):6166–6172. doi: 10.1021/ac301121z

Figure 3.

Figure 3

Dissociation of BCA from PEGA beads labeled with 4-carboxybenzene sulfonamide in the presence of seven soluble ligands, measured using MagLev. To measure these data, we first incubated PEGA beads in a solution of 450 µM BCA for one week. We then removed a batch of these beads (10 – 15 beads), rinsed them, and suspended them in a microcuvette containing the standard levitation buffer in which we had dissolved one of seven known ligands of BCA (the concentration of ligand is indicated on the graphs). We placed this microcuvette into our MagLev device and measured the change in levitation height over time (after allowing five minutes for the beads to reach their equilibrium levitation height). We used this data to calculate values for the association constant of each ligand with BCA by fitting these data to our RD model.