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. Author manuscript; available in PMC: 2013 Aug 17.
Published in final edited form as: Cell. 2012 Aug 2;150(4):780–791. doi: 10.1016/j.cell.2012.06.037

Figure 3. LPA and S1P activate YAP/TAZ by dephosphorylation.

Figure 3

HEK293A cells were treated with 1 μM LPA (A) or S1P (B) for the indicated times. Cell lysates were subjected to immunoblotting with the indicated antibodies. (C) Serum and LPA stimulate YAP interaction with TEAD1 but inhibit YAP interaction with 14-3-3. Cells were treated with LPA or serum as indicated. Cell lysates were subjected to immunoprecipitation (IP) with control IgG or YAP antibody. The co-immunoprecipitated TEAD1 and 14-3-3 were detected by immunoblotting. (D) LPA treatment (1 μM, for 1 h) induces YAP nuclear localization in HEK293A and MCF10A cells. Also see Figures S2 and S3.