Table 2. Purification of recombinant His-tagged AgT-DHDPS.
| Total protein (mg) | Total activity (U†) | Specific activity (U mg−1) | Yield (%) | Purification (fold) | |
|---|---|---|---|---|---|
| Lysate | 188 | 4238 | 23 | — | — |
| IMAC | 19 | 3326 | 175 | 78 | 8 |
| Size exclusion | 18 | 3125 | 174 | 74 | 8 |
1 U is defined as the consumption of 1 µmol NADPH per minute.