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. 2012 Aug 30;68(Pt 9):1040–1047. doi: 10.1107/S1744309112033052

Table 2. Purification of recombinant His-tagged AgT-DHDPS.

  Total protein (mg) Total activity (U) Specific activity (U mg−1) Yield (%) Purification (fold)
Lysate 188 4238 23
IMAC 19 3326 175 78 8
Size exclusion 18 3125 174 74 8

1 U is defined as the consumption of 1 µmol NADPH per minute.