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Contents and purity of the samples. (a) SDS–PAGE of samples fractionated with ammonium sulfate after x days of cultivation; x = 1, 3, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 and 20. Lane MW contains size markers (molecular weights are labelled in kDa). (b, c) The mass spectra of (b) a sample obtained after 7 d cultivation and (c) a sample purified by hydroxyapatite column chromatography after 20 d cultivation.
