Figure 2.

The binding of a proline-containing peptide (red) to the Src homology 3 domain (blue). Ligand binding stabilizes only those conformations that are binding-competent, inducing a redistribution in the conformational ensemble. The effects of binding can be mapped into a single molecule as shown on the right. The blue regions are the ones most affected by binding and the red regions the ones least affected. Notably, not all amino acids are predicted to be equally affected because of the lack of global cooperativity. A consequence of this result is that communication between distal sites involves only a subset of residues as elegantly shown by Pan et al. (22) for dihydrofolate reductase.