Table 1.
Methods for serum hormone analyses at the two laboratories (MGH and SFF).
| MGH assay details | |||||
|---|---|---|---|---|---|
| Hormone | Method | Manufacturer/System | Sensitivity | CVs | |
| Intra-assay | Inter-assay | ||||
| FSH | Microparticle Enzyme Immunoassay (MEIA) | Abbott AxSYM | 1.1 IU/L | 3–7% | 2–5% |
| LH | Microparticle Enzyme Immunoassay (MEIA) | Abbott AxSYM | 1.2 IU/L | 4–7% | 2–7% |
| Testosterone | Radioimmunoassay (RIA) | Coat-A-Count kit, Diagnostic Products Corp. | 0.14 nmol/L | 10% | 12% |
| SHBG | Solid-phase two-site enzyme chemiluminescent immunometric assay | Immulite, Diagnostic Products Corp. | 1 nmol/L | 2–5% | 4–8% |
| Inhibin B | Double antibody enzyme-linked immunosorbent assay (Double antibody ELISA) | Oxford Bioinnovation | 50 pg/mL | 8% | 20% |
| Estradiol | Microparticle Enzyme Immunoassay (MEIA) | Abbott AxSYM | 73 pmol/L | 3–11% | 5–15% |
| SFF assay details | |||||
|---|---|---|---|---|---|
| Hormone | Method | Manufacturer/System | Sensitivity | CVs | |
| Intra-assay | Inter-assay | ||||
| FSH | Time-resolved immunofluorometric assay (TR-IFMA) | DELFIA, Perkin Elmer | 0.05 IU/L | 1.3–2.1 % | 2.8–4.6 % |
| LH | Time-resolved immunofluorometric assay (TR-IFMA) | DELFIA, Perkin Elmer | 0.05 IU/L | 1.5–3.0 % | 4.0–4.5 % |
| Testosterone | Time-resolved fluoroimmunoassay (TR-FIA) | DELFIA, Perkin Elmer | 0.23 nmol/L | 1.4–2 % | 6–8 % |
| SHBG | Time-resolved immunofluorometric assay (TR-IFMA) | DELFIA, Perkin Elmer | 0.23 nmol/L | 3–5 % | 4–5 % |
| Inhibin B | Specific two-sided enzyme immunometric assay | (Oxford Bioinnovation, in-house standard) | 20 pg/ml | 15 % | 18 % |
| Estradiol | Radioimmunoassay (RIA) | Pantex, USA | 18 pmol/L | 3–8 % | 11–13 % |
CVs: coefficients of variation