Table II. Numbers of NKT cells and activated DCs (CD11c+) and mRNA levels of cytokines and chemokines in kidney after 24 h reperfusion from sham and ischemic mice injected with WT or S1pr3−/− BMDCs loaded with vehicle or α-GalCer.
| WT DC |
WT DC-α-GalCer |
S1pr3−/− DC |
S1pr3−/− DC-α-GalCer |
|||||
|---|---|---|---|---|---|---|---|---|
| Sham | IRI | Sham | IRI | Sham | IRI | Sham | IRI | |
| Immune cellsa | ||||||||
| NKT (×104) | 1.9 ± 0.5 | 2.2 ± 0.4 | 9.7 ± 0.6 | 15.1 ± 2.2*** | 0.9 ± 0.2 | 4.0 ± 1.1 | 5.6 ± 1.3 | 6.1 ± 1.3†† |
| CD11c+MHCII+ (×103) | 9.7 ± 3.4 | 38.4 ± 6.4 | 230 ± 84.5 | 1049.5 ± 294** | 7.8 ± 1.7 | 40.7 ± 18.5 | 246.4 ± 86 | 150 ± 54.5† |
| CD11c+CD40+ (×104) | 1.1 ± 0.2 | 1.5 ± 0.5 | 19.2 ± 66.1 | 86.9 ± 23.8** | 0.2 ± 0.1 | 2.1 ± 0.8 | 17.6 ± 4.6 | 12.6 ± 6.3†† |
| CD11c+CD1d+ (×103) | 0.4 ± 0.1 | 1.7 ± 0.5 | 55.9 ± 29.3 | 515.5 ± 125.9** | 0.3 ± 0.1 | 4.6 ± 2.6 | 89.3 ± 46 | 93.4 ± 51.7†† |
| mRNA levelsb | ||||||||
| TNF-α | 4.16 ± 0.67 | 1.04 ± 0.21 | 4.13 ± 0.64 | 0.74 ± 0.15 | ||||
| IL-12p40 | 3.24 ± 1.46 | 0.52 ± 0.14 | 0.22 ± 0.10 | 0.93 ± 0.67 | ||||
| IL-1β | 0.89 ± 0.40 | 3.17 ± 0.71** | 2.75 ± 0.67 | 0.69 ± 0.32 | ||||
| IL-6 | 10.21 ± 3.99 | 52.2 ± 82.81*** | 18.1 ± 9.35 | 0.46 ± 0.24 | ||||
| CXCL1 | 11.42 ± 3.56 | 18.63 ± 0.59 | 12.66 ± 1.37 | 1.52 ± 0.46 | ||||
| CXCL2 | 10.94 ± 4.86 | 84.45 ± 6.48** | 2.82 ± 0.51 | 0.51 ± 0.21 | ||||
| CXCL5 | 35.07 ± 13.43* | 61.40 ± 9.04* | 37.69 ± 15.02* | 6.32 ± 2.59 | ||||
a
For immune cells, total numbers of NKT (CD45+7-AAD−CD19−TCRβlowCd1dtet) and CD11c (CD45+7-AAD−CD11c+) cells as determined by flow cytometry are shown (n = 3–6/group). Data are means ± SEM. **p < 0.01, ***p < 0.001 compared with WT DC IRI; †p < 0.01, ††p < 0.001 compared with WT DC-α-GalCer IRI.
b
mRNA expression levels are shown relative to GAPDH and are expressed as fold ratios relative to respective sham-operated mice (n = 4–6/group). Data are means ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 relative to sham-operated mice.