Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2013 May 25.

Published in final edited form as: J Mol Biol. 2012 Mar 8;419(1-2):75–88. doi: 10.1016/j.jmb.2012.02.044

The mut1.2 surface patch is important for plasma membrane localization and polymerization of CHMP4B in vitro.

Confocal microscopy of CHMP4B(1-153)-flag localization in HEK293 cells.

(A) CHMP4B(1-153)-flag wild type, (B) CHMP4B(1-153)-flag carrying the mut1, (C) the mut2 and (D) the mut1.2 surface patch mutations. Individual confocal Z-sections are shown. Nuclei have been stained with 4′6-diamidino-2-phenylindole. The scale bar represents 10 μm.

(E) Sucrose gradient analyses of recombinant wild-type MBP-CHMP4B_ΔC-ALIX (top panel) and MBP-CHMP4B_ΔC-ALIX carrying the mut1.2 surface patch mutations (bottom panel). Molecular mass markers are indicated.

The mut1.2 surface patch is important for plasma membrane localization and polymerization of CHMP4B in vitro.

Confocal microscopy of CHMP4B(1-153)-flag localization in HEK293 cells.

(A) CHMP4B(1-153)-flag wild type, (B) CHMP4B(1-153)-flag carrying the mut1, (C) the mut2 and (D) the mut1.2 surface patch mutations. Individual confocal Z-sections are shown. Nuclei have been stained with 4′6-diamidino-2-phenylindole. The scale bar represents 10 μm.

(E) Sucrose gradient analyses of recombinant wild-type MBP-CHMP4B_ΔC-ALIX (top panel) and MBP-CHMP4B_ΔC-ALIX carrying the mut1.2 surface patch mutations (bottom panel). Molecular mass markers are indicated.