Skip to main content
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1974 Jan;1(1):97–103. doi: 10.1093/nar/1.1.97

The influence of subcellular fractions on the enzymic methylation of DNA in ascites cell nuclei

RH Burdon 1, JT Douglas 1
PMCID: PMC343327  PMID: 10793663

Abstract

Soluble factors appear to be present in both nuclei and cytoplasm of Krebs 2 ascites tumour cells capable of stimulating the enzyme catalysed methylation of DNA in isolated nuclei from these cells.

Full text

PDF
97

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BURDON R. H., SMELLIE R. M. Studies on the biosynthesis of ribonucleic acid in extracts of mammalian cells. Biochim Biophys Acta. 1961 Feb 12;47:93–106. doi: 10.1016/0006-3002(61)90833-2. [DOI] [PubMed] [Google Scholar]
  2. BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Burdon R. H. Enzymic modification of chromosomal macromolecules. I. DNA and protein methylation in mouse tumour cell chromatin. Biochim Biophys Acta. 1971 Mar 11;232(2):359–370. [PubMed] [Google Scholar]
  4. Burdon R. H., Martin B. T., Lal B. M. Synthesis of low molecular weight ribonucleic acid in tumour cells. J Mol Biol. 1967 Sep 14;28(2):357–371. doi: 10.1016/s0022-2836(67)80015-9. [DOI] [PubMed] [Google Scholar]
  5. Burdon R. H., Pearce C. A. Enzymic modification of chromosomal macromolecules. 3. The effect of adenine nucleotides on histone modification in chromatin and a possible influence of steroid hormones. Biochim Biophys Acta. 1971 Sep 24;246(3):561–571. [PubMed] [Google Scholar]
  6. Drahovský D., Morris N. R. Mechanism of action of rat liver DNA methylase. II. Interaction with single-stranded methyl-acceptor DNA. J Mol Biol. 1971 Oct 28;61(2):343–356. doi: 10.1016/0022-2836(71)90384-6. [DOI] [PubMed] [Google Scholar]
  7. Gefter M., Hausmann R., Gold M., Hurwitz J. The enzymatic methylation of ribonucleic acid and deoxyribonucleic acid. X. Bacteriophage T3-induced S-adenosylmethionine cleavage. J Biol Chem. 1966 May 10;241(9):1995–2006. [PubMed] [Google Scholar]
  8. Kalousek F., Morris N. R. Deoxyribonucleic acid methylase activity in rat spleen. J Biol Chem. 1968 May 10;243(9):2440–2442. [PubMed] [Google Scholar]
  9. Kappler J. W. The 5-methylcytosine content of DNA: tissue specificity. J Cell Physiol. 1971 Aug;78(1):33–36. doi: 10.1002/jcp.1040780106. [DOI] [PubMed] [Google Scholar]
  10. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  11. Scarano E., Iaccarino M., Grippo P., Parisi E. The heterogeneity of thymine methyl group origin in DNA pyrimidine isostichs of developing sea urchin embryos. Proc Natl Acad Sci U S A. 1967 May;57(5):1394–1400. doi: 10.1073/pnas.57.5.1394. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sheid B., Srinivasan P. R., Borek E. Deoxyribonucleic acid methylase of mammalian tissues. Biochemistry. 1968 Jan;7(1):280–285. doi: 10.1021/bi00841a034. [DOI] [PubMed] [Google Scholar]
  13. Vanyushin B. F., Mazin A. L., Vasilyev V. K., Belozersky A. N. The content of 5-methylcytosine in animal DNA: the species and tissue specificity. Biochim Biophys Acta. 1973 Mar 28;299(3):397–403. doi: 10.1016/0005-2787(73)90264-5. [DOI] [PubMed] [Google Scholar]
  14. Vanyushin B. F., Tkacheva S. G., Belozersky A. N. Rare bases in animal DNA. Nature. 1970 Mar 7;225(5236):948–949. doi: 10.1038/225948a0. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES