Table 1.
Primer design for fragment length analysis
| SNP | Sequence (5′ → 3′) | PCR product (bp) | Annealing temperature (°C) | Restriction enzyme |
|---|---|---|---|---|
| rs10934657 |
TGGCAAGAGGGAGAGGAG |
139 |
55.2 |
AluI |
| |
CTTCCTCCTCTGTAAACCAGAGAGA |
|
|
|
| rs111472457 |
CATCCGAGATGCAAGACCTAGA |
156 |
58.5 |
AluI |
| |
CCGTGAGGGATTCGGAGT |
|
|
|
| rs35057827 |
GCATGAGGTGTTACATCACCAGCCAC |
123 |
60.9 |
BtgI |
| |
CAATCCAGTCCAACACCTGCT |
|
|
|
| rs61746078 |
CAGCAGGATGTACAGCAGGT |
150 |
61.2 |
AluI |
| |
GTACGTATTCTGAGCCACAG |
|
|
|
| rs13074913 |
TCTACAAGGCAGCTCGACAC |
136 |
58 |
MscI |
| |
AGGTCTTCCATCCATGGCC |
|
|
|
| rs61745397 |
GCCAGGGACTCGGCTGGA |
154 |
60.6 |
BamHI |
| |
TCACCTCGATGGTGTACTGC |
|
|
|
| rs112304715 |
CAGCAGGGACAGGATCTGCACT |
170 |
62.5 |
SpeI |
| |
AGCCGCTTATGAGTCTGCTCT |
|
|
|
| rs77832285 |
TCCTGAGTGAGCTCCTGCATAT |
110 |
59 |
NdeI |
| |
GCTCGAACACCACATATTGC |
|
|
|
| rs2289838 |
AGCCCGGAAGAAAGAATTTA |
168 |
58.2 |
AluI |
| |
TGGATGTTGCCAAAGATGATAAG |
|
|
|
| rs74389479 |
AAGTACGAGCAACTGCCTGAG |
138 |
59.5 |
AluI |
| |
ATCAAAGAAGGTGCCCGCAA |
|
|
|
| rs1062749 |
CTGCAAATTCGCCTTGTGGT |
162 |
55.2 |
SacI |
| GCTGAAGTGGCTCCTTTAGAGCT |
Mismatch positions are highlighted in italics.