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. Author manuscript; available in PMC: 2013 Aug 22.
Published in final edited form as: J Am Chem Soc. 2012 Aug 9;134(33):13692–13699. doi: 10.1021/ja303374p

Figure 6.

Figure 6

Comparative studies of labeling with noncovalent TMP-tag and covalent TMP-tag. (A) HEK 293T cells expressing Tomm20-eDHFR were labeled with TMP-Fl as in Figure 5A. After labeling, cells were imaged using confocal microscope (Live panel) or fixed with 4% paraformaldehyde in PBS for 10 min followed by washing with PBS for 12 h before imaged (Fixed panel). (B) HEK 293T cells expressing Tomm20-eDHFR:L28C were labeled with A-TMP-Fl and examined with and without fixation treatment as in (A). Scale bars are 25 μ m.