Table 2.
Band assignments for bacterial injury from diallyl sulfide and garlic extract
| Bacteria | Antimicrobials | Cellular Structures Affected | FTIR Band Assignment | Raman Band Assignment | ||
|---|---|---|---|---|---|---|
| Proteins | amide I of α-helical structure | 1655 cm−1 | S-S disulfide stretch in proteins | 524 cm−1 | ||
| amide II | 1545 cm−1 | v(S-S) trans-gauche-trans (amino acid cysteine) | 540 cm−1 | |||
| sym bending modes of methyl groups in skeletal proteins | 1455 cm−1 | C-C twist aromatic ring | 620 cm−1 | |||
| asym CH3 bending of the methyl groups of proteins | 1449 cm−1 | C-S stretch mode of cysteine | 662 cm−1 | |||
| sym CH3 bending modes of the methyl groups of proteins | 1401 cm−1 | v(C-S) trans (amino acid cysteine) | 700 cm−1 | |||
| sym breathing of tryptophan | 756 cm−1 | |||||
| Diallyl sulfide | phenylalanine | 1008 cm−1 | ||||
| tyrosine | 1373 cm−1 | |||||
| E. coli O157:H7 | phenylalanine | 1582 cm−1 | ||||
|
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| Lipids | CH2 scissoring mode of the acyl chain of lipids | 1465 cm−1 | phosphatidylinositol | 596 cm−1 | ||
| sym stretch of phosphate groups in phospholipids | 1224 cm−1 | phospholipid | 1130 cm−1 | |||
| sym phosphate stretching mode of phosphodiesters | 1080 cm−1 | |||||
| phosphodiester | 916 cm−1 | |||||
|
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| Nucleic | acids asym phosphate stretch from phosphodiester groups of cellular nucleic acids | 1241 cm−1 | ND | |||
| C-O ribose and C-C | 994 cm−1 | |||||
|
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| Phenolics | Proteins | C-O stretch mode of C-OH groups of serine, threonine, and tyrosine | 1164 cm−1 | ND | ||
| amide III | 1309 cm−1 | |||||
|
| ||||||
| amide I of α-helical structure | 1655 cm−1 | S-S disulfide stretch | 509 cm−1 | |||
| Proteins | amide I of β-sheet structures | 1637 cm−1 | v(S-S) trans-gauche-trans (amino acid cysteine) | 540 cm−1 | ||
| Diallyl sulfide | amide II | 1545 cm−1 | C-S stretch of proteins | 640 cm−1 | ||
|
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| Lipids | phosphodiester | 916 cm−1 | ND | |||
|
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| Nucleic acids | asym phosphate stretch from phosphodiester groups of cellular nucleic acids | 1241 cm−1 | ND | |||
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| L. monocytogenes | Proteins | amide III | 1309 cm−1 | |||
| sym CH3 bending modes of methyl groups of proteins | 1401 cm−1 | ND | ||||
|
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| Phenolics | Lipids | ND | C=O stretch | 1762 cm−1 | ||
|
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| Polysaccharides | ND | saccharide | 1113 cm−1 | |||
| ring structures | 1416 cm−1 | |||||
*
note: sym: symmetric; asym: antisymmetric; ND: non detected