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. 2012 Sep 4;7(9):e44371. doi: 10.1371/journal.pone.0044371

Figure 1. Effects of PRL on hippocampal precursors in vitro and in vivo.

Figure 1

The addition of exogenous PRL led to a significant increase in hippocampal (A) derived neurospheres (n = 5 experiments). As well as increasing hippocampal neurosphere number, exposure of primary hippocampal cells to exogenous PRL resulted in an increase in small and medium sized neurospheres with diameters between 40 and 150 µm (B). Following differentiation, the large KCl-activated neurospheres contained both neurons (red) and astrocytes (green) (C) whereas; the smaller PRL- and control-cultured neurospheres gave rise to GFAP-positive astrocytes exclusively (D). Infusion of PRL directly into the adult hippocampus for 7 days resulted in a significant increase in hippocampal neurosphere numbers (E). Results are expressed as mean ± SEM. *p<0.05, **p<0.01. Scale bars are 20 µm.