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. 2012 Sep 4;7(9):e44688. doi: 10.1371/journal.pone.0044688

Figure 2. Effects of different sizes of TiO2-NPs on ROS production and expression patterns of genes controlling oxidative stress in nematodes.

Figure 2

(A–B) Comparison of ROS production in nematodes exposed to different sizes of TiO2-NPs. (C) Expression patterns of genes controlling oxidative stress in nematodes exposed to different sizes of TiO2-NPs at the concentration of 10 µg/L. (D) Expression patterns of sod-2 gene in nematodes exposed to different sizes of TiO2-NPs. (E) Expression patterns of sod-3 gene in nematodes exposed to different sizes of TiO2-NPs. Relative expression ratios (between target genes and act-1 reference gene) in treatments were normalized to the control. Exposure of TiO2-NPs was performed from L1-larvae, and the endpoints were examined when nematodes developed into the adults. Ti-NPs, TiO2-NPs. The Bars represent mean±S.E.M. *p<0.05, **p<0.01.