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. 2012 Sep 4;7(9):e43688. doi: 10.1371/journal.pone.0043688

Figure 4. Immunohistochemistry of VAMP8 and Rab3D.

Figure 4

(A), (B): VAMP8 expression in normal controls was localized to the apical membrane of the lacrimal gland epithelia (A, B; 87 years old, Female). (C), (D): In the VDT group, strong VAMP8 immunostaining was present in the cytoplasm and basal side of the lacrimal gland epithelial cells but not at the apical membrane (C, D; VDT-case 3). (E), (F): VAMP8 staining in the SS group was seen close to the basal side of the acinar epithelia (E, F; SS-case 8). A, C, E; Original magnification: ×400, B, D, F; Magnified view of the acinus with asterisk in A, C, E. Scale bars = 20 µm. (G), (H): Rab3D expression in normal controls. Rab3D was expressed at the apical side of the cytoplasm in the lacrimal gland epithelia, but not at the apical membrane, where VAMP8 was expressed (G, H; 87 years old, Female). (I), (J): In the VDT group, strong Rab3D immunostaining was observed in the cytoplasm of the lacrimal gland epithelial cells (I, J; VDT-case 3). (K), (L): In the SS group, faint Rab3D expression was observed at the basal side of the acinar epithelia (K, L; SS-case 8). G, I, K; Original magnification: ×400. H, J, L: Magnified view of the acinus with asterisk in G, I, and K. Scale bars = 20 µm.