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. 2012 Sep 4;7(9):e43824. doi: 10.1371/journal.pone.0043824

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© 2012 Liu et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Lentiviral vector-mediated silencing of endogenous p53 in replicon cells (JFH1/HepG2) treated with the indicated concentration of ribavirin for 48 h. (A) The expression of total p53, phosphorylated p53 at Ser15 (p53ser15∼pi) and HCV NS3 viral proteins was analyzed by immunoblotting. The Western blot was independently repeated five times and one representative result was shown here. The band intensity of each protein in each experiment was determined by densitometry. The expression level change of proteins were calculated by dividing the band intensity of the indicated proteins, normalized to that of the loading control β-actin, with that observed in the samples receiving scramble-shRNA treatment. (B) HCV RNA levels were measured by quantitative RT-PCR. Each result represents the mean ± s.e.m. of 5 independent determinations and is considered significant at P<0.05. RBV: ribavirin.