The sequence logo of the PxRxRN motif was derived from 174 tRNase ZLs from 75 fungi, 21 plants and 68 metazoans. The sequence log of the PxPxRG motif is derived from alignment of 44 metazoan tRNase ZSs. The sequence logo of the AxDx motif is derived from 240 alignments including 178 tRNase ZLs from 71 metazoan, 21 green plants, 77 fungi, and 62 tRNase ZSs from 30 metazoans, 9 fungi, 2 bacteria and M. brevicollis. The sequence logos were created using WebLogo (http://weblogo.berkeley.edu). The height of each amino acid indicates the level of conservation at that position. Amino acids are colored as follows: red, basic; blue, acidic; orange, polar; and green, hydrophobic.