Figure 7. Growth complementation analysis of S. pombe Trz1 with a mutation in the AxDx motif.

A S. pombe strain bearing a temperature-sensitive allele of trz1 (trz1-1) was transformed with empty vector (4X) or vector expressing the wild-type Trz1 or the Trz1D772A mutant, and equal amounts of cells were spotted on EMM medium supplemented with leucine and adenine and grown at 26°C or 37°C.