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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: Transgenic Res. 2012 Feb 15;21(5):1117–1123. doi: 10.1007/s11248-012-9598-y

Fig. 1.

Fig. 1

Diagram of the strategy generating the K8–CreERT2 targeting BAC clone. Transgene, including CreERT2 cDNA, an IRES–GFP expression cassette, and the Zeocin resistant gene, was inserted at the AUG translational initiation codon of the K8 gene in BAC clone RP23-234D2. Zeocin serves as a positive selection marker during BAC recombineering. IRES internal ribosomal entry site. GFP green fluorescence protein