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. Author manuscript; available in PMC: 2013 Sep 4.
Published in final edited form as: Biochemistry. 2012 Aug 21;51(35):6950–6960. doi: 10.1021/bi3008033

FIGURE 4.

FIGURE 4

Localization of GIP and a GIP-binding peptide ESSVDLLDG in D54 MG human glioma cells (A) Internalization of the peptide by glioma cells. The peptide was labeled with TAMRA and is shown inside the cells as red fluorescence. (B) ESSVDLLDG peptide was localized in the cytoplasm of the glioma cell (red). (C) GIP was localized in the cytoplasm of the same cell using primary anti-GIP antibody followed by secondary antibody conjugated to Alexa 488 (green). (D) Merged image of (B) and (C) showing colocalization (indicated with arrows) of GIP and ESSVDLLDG peptide within the cell. Cell nuclei were stained with DAPI (blue).