Normalized FTIR ATR spectra of unstretched plasma clot (black line) and the same clot after twofold (strain = 1, green solid line), threefold (strain = 2, red solid line), and fourfold (strain = 3, blue solid line) elongation. Corresponding difference spectra (1-0, 2-0, and 3-0) obtained by subtraction of the initial spectrum at strain = 0 are shown as the dashed lines of the corresponding color. (Inset) The absorbance intensity ratio at 1622/1651 cm−1 as a function of strain. Strain is defined as stretched length/initial length-1.