Figure 5.

Reversibility of the deformation-induced changes in FTIR ATR spectra. (A) The average ratio of second derivative of absorbance intensities at 1622/1651 cm⁻¹ in unstretched, stretched at strains 1 (n = 9) and 2 (n = 4), and relaxed fibrin clots. (Inset) Relative reversibility of the average ratio of absorbance intensities at 1622/1651 cm⁻¹ as a function of strain. The reversibility was calculated as 100^∗(average value in the stretched state – average value after relaxation)/(average value in the stretched state – average initial value). (B) The average ratio of second derivative of absorbance intensities at 1622/1651 cm⁻¹ in uncompressed, compressed, and decompressed fibrin clots (n = 10) at various pressures. (Inset) The residual ratio of absorbance intensities at 1622/1651 cm⁻¹ in individual decompressed fibrin clots plotted against the ratio measured in the same fibrin clots at the peak of compression. Error bars represent standard deviations.