Table 2.
Association kinetics of WT TEM1 with BLIP D163K in buffer and crowded solutions
| Solution | Relative viscosity | K1 = k−1/k1 (μM) | k2 (s−1) | kon = k2/K1 (M−1 s−1) |
|---|---|---|---|---|
| Buffer | 1 | 58 (13) | 64 (8) | 11 × 105 |
| 15% dextran | 10.6 | 31 (3) | 59 (3) | 19 × 105 |
| 4% PEG | 4 | 55 (20) | 77 (19) | 14 × 105 |
| 8% PEG | 9 | 43 (10) | 63 (7) | 15 × 105 |
| 12% PEG | 19.4 | 47 (13) | 53 (10) | 11 × 105 |
Relative viscosities were measured using a Cannon-Fenske Routine Viscometer. K1 and k2 were extracted from the fit to Eq. 2. Standard errors are in parentheses.