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Flow chart for image processing with our polar fitting technique (left) and the oval profile technique (right), showing the reduction in the number of essential manual steps that may lead to error. Experimental steps (open), automatic steps (dark-shaded), and manual steps (light-shaded) result in the desired output (solid). (In each case, the dashed box represents the software used to process the image; steps outside of the dashed box require manual processing.)
