Table 1.

Order parameters for di-4-ANEPPS in GUVs found by three image-processing methods and the error associated with each technique for both analysis of one image and for a batch of images (n = 7)

	One image		Twelve images
	ϑ1PF	± Error	ϑ1PF	± Error
Oval profile	0.729	0.019 (2.6%)	0.747	0.078 (10.4%)
Polar manual	0.715	0.0027 (0.4%)	0.725	0.018 (2.5%)
Polar semiautomated	0.716	0.0013 (0.2%)	0.727	0.018 (2.5%)