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. 2012 Sep 5;103(5):851–859. doi: 10.1016/j.bpj.2012.06.036

Figure 1.

Figure 1

TCs are transcription-dependent compartments in the nucleus. (A and B) Nuclear Alexa546-UTP (red)-enriched TCs colocalized with Alexa488 antibody (green) against active RNA pol II (phosphorylated S5) in fixed untreated HeLa cells (A) and in α-amanitin-treated cells (20 μg/ml for 1 h) with DNA marked with Hoescht (blue) (B). DNA was marked by Hoescht to determine the nuclear boundary. (C) Quantification of TCs using Alexa546-UTP with and without 1 h treatment of α-amanitin (20 μg/ml) for N ∼ 30 cells. (D) Quantification of colocalization fraction between active RNA pol II and Alexa546-UTP in untreated 1 h treatment of α-amanitin (20 μg/ml) or DRB (40 μM). ∗P < 0.05. Scale bar, 5 μm.