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. 2012 Sep 5;103(5):851–859. doi: 10.1016/j.bpj.2012.06.036

Figure 2.

Figure 2

UTP uptake by TCs depends on the transcription state. (A) Collage of the nuclear TCs and the cytoplasmic UTP foci labeled with Cy5-UTP, which was sequentially photobleached with a 635 laser. The color bar shows the intensity normalized for the complete time series. (B) Graph of the mean intensity of Cy5-UTP in TCs normalized to the first frame of imaging plotted for TCs in nucleus in control and α-amanitin-treated cells (20 μg/ml for 1 h). Intensities measured in the cytoplasmic mitochondrial aggregates of UTP were insensitive to α-amanitin treatment.