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. Author manuscript; available in PMC: 2013 Dec 28.
Published in final edited form as: Cancer Lett. 2012 Jul 7;325(2):139–146. doi: 10.1016/j.canlet.2012.06.018

Fig. 5. Effects of BKM120 and RAD001 combination on Akt phosphorylation, mTOR signaling and p21 expression.

Fig. 5

The indicated cell lines were plated in 10 cm-diameter cell culture dishes and treated next day with 1 nM RAD001 in the absence and presence of 1 μM BKM120 for 24 h. The cells were then harvested for preparation of whole-cell protein lysates and subsequent Western blot analysis to detect the indicated proteins.