Figure 6.

Dose-response curves of the inhibitors against SaV protease. The inhibitory effects of the three chemical compounds that were screened for their structural similarity to the authentic substrates of SaV protease were determined with an in vitro trans cleavage assay. A radiolabeled full-length Mc10 ORF1 polyprotein containing a defective protease (Pro^mut; Oka et al., 2005b) or a non-radiolabeled partial Mc10 ORF1 polyprotein (NS6-7-VP1) containing a functional protease (Pro^wt; Oka et al., 2006) was separately expressed using the in vitro transcription/translation system. The translation products were mixed and incubated in the presence of increasing concentrations of the indicated compounds at 30°C for 20 h. The intensity of the radioactive band corresponding to the NS4-NS5 product was measured with Typhoon 7500 and plotted in relation to the compound concentrations. (A) Compound No.50. (B) Compound No.100. (C) Compound No.116.